CA2262618A1 - Novel boronic acid-containing nucleic acids and their use as diagnostic agents - Google Patents

Novel boronic acid-containing nucleic acids and their use as diagnostic agents

Info

Publication number
CA2262618A1
CA2262618A1 CA002262618A CA2262618A CA2262618A1 CA 2262618 A1 CA2262618 A1 CA 2262618A1 CA 002262618 A CA002262618 A CA 002262618A CA 2262618 A CA2262618 A CA 2262618A CA 2262618 A1 CA2262618 A1 CA 2262618A1
Authority
CA
Canada
Prior art keywords
boronic acid
nucleic acids
diagnostic agents
containing nucleic
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002262618A
Other languages
French (fr)
Other versions
CA2262618C (en
Inventor
Mark L. Stolowitz
Robert J. Kaiser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambrex Bio Science Rockland Inc
Original Assignee
Prolinx, Inc.
Mark L. Stolowitz
Robert J. Kaiser
Agilent Technologies, Inc.
Cambrex Bio Science Rockland, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prolinx, Inc., Mark L. Stolowitz, Robert J. Kaiser, Agilent Technologies, Inc., Cambrex Bio Science Rockland, Inc. filed Critical Prolinx, Inc.
Publication of CA2262618A1 publication Critical patent/CA2262618A1/en
Application granted granted Critical
Publication of CA2262618C publication Critical patent/CA2262618C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided.
The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.
CA002262618A 1996-08-05 1997-07-31 Novel boronic acid-containing nucleic acids and their use as diagnostic agents Expired - Fee Related CA2262618C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/692,429 US5831046A (en) 1996-08-05 1996-08-05 Boronic acid-contaning nucleic acid monomers
US08/692,429 1996-08-05
PCT/US1997/012834 WO1998005672A1 (en) 1996-08-05 1997-07-31 Novel boronic acid-containing nucleic acids and their use as diagnostic agents

Publications (2)

Publication Number Publication Date
CA2262618A1 true CA2262618A1 (en) 1998-02-12
CA2262618C CA2262618C (en) 2007-10-02

Family

ID=24780549

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002262618A Expired - Fee Related CA2262618C (en) 1996-08-05 1997-07-31 Novel boronic acid-containing nucleic acids and their use as diagnostic agents

Country Status (6)

Country Link
US (3) US5831046A (en)
EP (1) EP0923591A4 (en)
JP (1) JP2000515546A (en)
AU (1) AU729907B2 (en)
CA (1) CA2262618C (en)
WO (1) WO1998005672A1 (en)

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Also Published As

Publication number Publication date
AU3809097A (en) 1998-02-25
AU729907B2 (en) 2001-02-15
EP0923591A1 (en) 1999-06-23
US5876938A (en) 1999-03-02
US5831046A (en) 1998-11-03
US5831045A (en) 1998-11-03
CA2262618C (en) 2007-10-02
JP2000515546A (en) 2000-11-21
WO1998005672A1 (en) 1998-02-12
EP0923591A4 (en) 2001-01-31

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